GARLIC AND TEA TREE OIL COMPOUNDS PROMOTE OXIDATION OF THE TYPICAL 2-CYS PEROXIREDOXIN PROTEINS IN JURKAT T-LYMPHOCYTES
Journal of Basrah Researches (Sciences),
Volume 45, Issue 1, Pages 65-76
AbstractThe mammalian typical 2-Cys peroxiredoxin (Prx) proteins have emerged as important sensors and transducers in the cellular response to oxidative stress. Plant natural products have been shown to have both pro- and anti-oxidant effects but their exact mechanisms of action are not fully understood. Here we show that selected compounds from garlic and tea tree oils promote either oxidised dimer formation or hyperoxidised monomer formation in the typical 2-Cys Prx proteins. Diallyl disulphide (DADS) and diallyl trisulphide (DATS) were the main constituents of garlic oil and showed similar effects to their parent oil but DATS was more effective than DADS that indicating to the importance of the number of sulphur atoms. Tea tree oil was a less potent promotor of the oxidation of the Prx proteins than garlic oil. In contrast, terpinen-4-ol, the major constituent of tea tree oil, was a very effective promotor of the oxidation of the Prx proteins. We show that the results could partly, but not entirely, be explained by differences in the effects of the tested compounds on the activity of thioredoxin reductase (TrxR), the enzyme required for the regeneration of the reduced monomer form of the Prx proteins from their oxidised dimer form. The results are discussed in terms of the involvement of the typical 2-Cys Prx proteins in the cellular response oxidative stress.
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