ISSN: 1817-2695

Volume 32, Issue 2

Volume 32, Issue 2, Spring 2006, Page 1-41

The Effect of Some Fungi in Biocontrol in Two Spotted Spider Mite Tetranychus urticae (Koch.) (Tetranychidae : Acari)

H. M. R. Mehdi

Journal of Basrah Researches (Sciences), Volume 32, Issue 2, Pages 12-26

The following fungi Alternaria alternata, A.pluriseptata, Aspergillus terreus, Eurotium eurotiorum, Stachybotrys atra, Ulocladium chartarum were isolated from the nymphal and adult instars of two-spotted spider mite. Fungal inoculum of 106 spores/ml revealed a rate of mortality 45.9, 42.03% on the egg mites for both S.atra, Trichoderma harzianum, respectively. The spore suspension of A.terreus on nymphal instars showed rate of mortality 63.03% after 72 hourse of treatment. It was also shown that the spore suspension of A.terreus, and T.harzianum revealed highest rate of adult instars mortality 65.5, 62.9% respectively. The effect of culture filterate of A.terreus and A.alternata on egg instars mortality was 55.9, 53.1% respectively. An increase of fungal culture filtrate in the media was showed an increase in the rate of mortality of nyphal instars. Apparently that the culture filtrate of A.alternata ; A.pluriseptata and A.terrens revealed mortality rates of 46.2, 45.5, 44.4% respectively. The rate of mortality in the culture filterate on adult instars 45.8, 45.01% by A.terreus and A.alternata respectively.

Improve production of Indole-3- acetic acid From two mutant isolates of Trichoderma harzianum by UV. radiation

Mohammed H. Abass And Mohammed A. Hameed

Journal of Basrah Researches (Sciences), Volume 32, Issue 2, Pages 27-37

The present study has been performed at Date Palm Research Center and Agricultural college laboratories to determine the ability of different isolates of Trichoderma harzianum to produce Indole-3-acetic acid(IAA), and enhance their productivity. Two wild isolates (T2 and T7) showed an ability to produce IAA at levels of 1.77 and 33.24 ppm, respectively. T2 wt. isolate was selected and improved by exposing it to UV radiation. The results of chromatography analysis (by using HPLC) revealed the enhancement production of IAA in the fungal filtrates of mutant T2mut. 75 and T2mut. 90 , which were 65.50 and 47.72 ppm, respectively. This enhancement correlated positively with the bioassay of Wheat and Corn seeds, the seedlings viability index (cm) increased significantly when treated with colony filtrates of mutants. Results proved the tolerance of two mutants to the high concentration of Benlate fungicide reached 1000 ppm, in contrast with susceptible wild isolates of T. harzianum .

Impact of Electrical Current (A.C.) on Chemical Composition of Four Fish Species and Required Lethal Voltages

Batool H. H. Al-Mhnawi

Journal of Basrah Researches (Sciences), Volume 32, Issue 2, Pages 38-41

Four fish species i.e. fresh water mullet Liza abu, grass carp Ctenopharyngodon idella, common carp Cyprinus carpio and yellowtail bream Acanthopagrus latus were subgected to electrical current type AC. The effective voltage for the four species ranged between 20-480V, while lethal voltage was 140-500V. the yellowtail bream need less time and voltage compared to the rest species followed by mullet, grass carp where the last two showed no significant difference while common carp recorded the highest values for both effective and lethal voltages. Significant correlation coefficient (r = 0.49) were found between effective voltage and time, also regrrsion coefficient were determined for the four species. The chemical composition before and after exposure to lethal voltage showed that fat and protein decrease in mullet and bream respectively. The present study rivaled the possibility of use electrical current for anesthetic fishes.